Figure 1.
![Figure 1. Deficiency of p85α in BMMs results in reduced proliferation. Nonadherent bone marrow cells (1 × 106) from WT and p85α–/– mice were cultured in a 24-well tissue-culture plate in the presence of M-CSF (100 ng/mL) for 3 days. Cells were starved overnight using IMDM and stimulated with either M-CSF (100 ng/mL) or GM-CSF (50 ng/mL) for 24 hours. Proliferation was measured by thymidine incorporation assay. Bars represent the mean thymidine incorporation in BMMs in response to either M-CSF or GM-CSF stimulation (counts per minute [cpm] ± SD) of 2 experiments performed in duplicate. Similar results were observed in 3 independent experiments. *P<.05 for WT versus p85α–/–.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/1/10.1182_blood-2004-10-4041/4/zh80130580760001.jpeg?Expires=1724241674&Signature=BvCDprSEA5RpHzKl9hrfDOkwOs1cTJhyZnUqJDJWeVTjB0lI7iZyw46ITY-CCYNF79JBxj9CsL4Zhw23yLa6R7v2Bt1LVVafia~qff7VcGgkbLLQsG0T9vHQp8ZoRCmSXO0eapMSFHxR7GYp7PEXu2XX4GSh9IEenfHVG619Ur5OI~NRiXfXbyvNviJUv5Sf3Mgq8hUSlgmpFYcKBAyf~XtDM-NV011VCb3dRlCChvFK5YRxBGuO5fMPIAKTK1WJqTVf96vcD3sWwHmD2PKd0~HK5JNdmOTrh1rQ76rl5mZhxKEQYFoP-ZFHxo~4SLPuTlTx0cxE2hJL~gG4tKjxkQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Deficiency of p85α in BMMs results in reduced proliferation. Nonadherent bone marrow cells (1 × 106) from WT and p85α–/– mice were cultured in a 24-well tissue-culture plate in the presence of M-CSF (100 ng/mL) for 3 days. Cells were starved overnight using IMDM and stimulated with either M-CSF (100 ng/mL) or GM-CSF (50 ng/mL) for 24 hours. Proliferation was measured by thymidine incorporation assay. Bars represent the mean thymidine incorporation in BMMs in response to either M-CSF or GM-CSF stimulation (counts per minute [cpm] ± SD) of 2 experiments performed in duplicate. Similar results were observed in 3 independent experiments. *P<.05 for WT versus p85α–/–.
