Figure 2.
![Figure 2. Erythroid differentiation is inhibited in human CD34+ cells expressing RPS19 shRNAs. In panel A, mean glycophorin A (Gly-A) expression, assayed by flow cytometry, is shown as a percentage of the luciferase (luc) shRNA control (mean and SEM). In panel B, mean CD11b expression is displayed as a percentage of the control. Methylcellulose colony assays were performed with cells expressing control or RPS19 shRNAs. The number of BFU-E colonies is presented in panel C, and the number of CFU-GM colonies is presented in panel D (mean [SEM]). *P < .001 by 2-tailed Student t test; **P < .05.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/12/10.1182_blood-2004-08-3313/5/zh80120579690002.jpeg?Expires=1722774644&Signature=Gi5xLkjUJGL6uNVoW10g0oMt4f3KeKhWWKl-f1XmTMUPtiITJJk-y7-J1EZALK667HzmsOwi74qnn0IIwKeC-L1Te38xOmRxSoTXSn3mJKQNTYWlH4WzXdcHk1nl1S~Jvps22XNgXeP~WqlGOeXWZrbTWhrgdYXBSVCgh4HegaBwJQtO193NipqeCIwnm5edffp~1K3o91gfFVVK6WJrQRv6nWcLVCAk3JG54Y~tM-tIdoGOzgwRskGc5vrPeQ9RSgw9WL45e1gC2JAdK2le0~WQvKOpXZDKtQa5chUNmq4LUrlN7vmbmoJs28wAS~xqVjCH~JLMs-JvmrdZA~4Zmg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Erythroid differentiation is inhibited in human CD34+cells expressing RPS19 shRNAs. In panel A, mean glycophorin A (Gly-A) expression, assayed by flow cytometry, is shown as a percentage of the luciferase (luc) shRNA control (mean and SEM). In panel B, mean CD11b expression is displayed as a percentage of the control. Methylcellulose colony assays were performed with cells expressing control or RPS19 shRNAs. The number of BFU-E colonies is presented in panel C, and the number of CFU-GM colonies is presented in panel D (mean [SEM]). *P < .001 by 2-tailed Student t test; **P < .05.
