American Society of Hematology

Figure 1.

$Figure 1. Mature-DC– and immature-DC–derived exosomes are qualitatively similar. (A) Whole-mount exosomes observed by electron microscopy. Bar represents 100 nm. Arrows indicate some examples of immature exosomes (imm exo) or mature exosomes (mat exo). (B) Immature (imm exo) or mature exosomes (mat exo) were subjected to flotation on a continuous sucrose gradient. Twelve fractions were collected, run on a 10% SDS gel, and analyzed by Western blot. Staining for MHC class II molecules is shown. (C) Coomassie blue staining of 30 μg immature (imm) and mature (mat) exosomes separated on 4% to 12% SDS gel.$

Mature-DC– and immature-DC–derived exosomes are qualitatively similar. (A) Whole-mount exosomes observed by electron microscopy. Bar represents 100 nm. Arrows indicate some examples of immature exosomes (imm exo) or mature exosomes (mat exo). (B) Immature (imm exo) or mature exosomes (mat exo) were subjected to flotation on a continuous sucrose gradient. Twelve fractions were collected, run on a 10% SDS gel, and analyzed by Western blot. Staining for MHC class II molecules is shown. (C) Coomassie blue staining of 30 μg immature (imm) and mature (mat) exosomes separated on 4% to 12% SDS gel.

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