American Society of Hematology

Figure 4.

$Figure 4. The Lnk SH2 domain is required to inhibit Epo-dependent erythroblast survival in in vitro culture. Ter119- fetal liver erythroid progenitor cells were purified, infected, and cultured as described in “Materials and methods.” We then introduced either the control vector or the wild-type or mutant Lnk cDNA constructs into Ter119- erythroid progenitors. (A) Average cell numbers (± SD) at the end of 48-hour culture. The numbers were normalized to the starting culture density of 1 × 105/mL. (B) At 40 hours after initiation of the culture, the erythroblasts were stained for annexin V and 7-AAD, and subjected to FACS analysis. hCD4+ fractions (ie, infected cells) were gated in all the FACS plots. The apoptotic cell fractions (annexin V+ and 7-AAD-) and dead cell fractions (annexin V+ and 7-AAD+) are indicated in each panel.$

The Lnk SH2 domain is required to inhibit Epo-dependent erythroblast survival in in vitro culture. Ter119^- fetal liver erythroid progenitor cells were purified, infected, and cultured as described in “Materials and methods.” We then introduced either the control vector or the wild-type or mutant Lnk cDNA constructs into Ter119^- erythroid progenitors. (A) Average cell numbers (± SD) at the end of 48-hour culture. The numbers were normalized to the starting culture density of 1 × 10⁵/mL. (B) At 40 hours after initiation of the culture, the erythroblasts were stained for annexin V and 7-AAD, and subjected to FACS analysis. hCD4⁺ fractions (ie, infected cells) were gated in all the FACS plots. The apoptotic cell fractions (annexin V⁺ and 7-AAD^-) and dead cell fractions (annexin V⁺ and 7-AAD⁺) are indicated in each panel.

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