Figure 2.
Hypersensitive CFU-GM colony growth induced by E76K SHP-2 is dependent on PTPase activity but does not require the βc subunit. (A) CFU-GM colony growth of fetal liver cells expressing WT SHP-2, WT-C463S (catalytically inactive WT SHP-2), E76K SHP-2, or E76K-C463S (a catalytically inactive E76K mutant SHP-2) over a range of GM-CSF concentrations. Cells expressing WT, WT-C463S, and E76K-C463S SHP-2 proteins all demonstrate a normal pattern of CFU-GM colony growth. (B) CFU-GM colony growth of WT and βc-deficient (βc-/-) bone marrow cells transduced with WT or E76K SHP-2 vectors over a range of IL-3 concentrations. The βc-/- bone marrow cells expressing E76K SHP-2 demonstrate a hypersensitive response that is similar to WT bone marrow cells. Error bars indicate the standard deviations of duplicate plates.

Hypersensitive CFU-GM colony growth induced by E76K SHP-2 is dependent on PTPase activity but does not require the βc subunit. (A) CFU-GM colony growth of fetal liver cells expressing WT SHP-2, WT-C463S (catalytically inactive WT SHP-2), E76K SHP-2, or E76K-C463S (a catalytically inactive E76K mutant SHP-2) over a range of GM-CSF concentrations. Cells expressing WT, WT-C463S, and E76K-C463S SHP-2 proteins all demonstrate a normal pattern of CFU-GM colony growth. (B) CFU-GM colony growth of WT and βc-deficient (βc-/-) bone marrow cells transduced with WT or E76K SHP-2 vectors over a range of IL-3 concentrations. The βc-/- bone marrow cells expressing E76K SHP-2 demonstrate a hypersensitive response that is similar to WT bone marrow cells. Error bars indicate the standard deviations of duplicate plates.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal