Effects of hypoxia-reoxygenation on LTBMC. (A) LTBMC was established for BM cells that were untreated (Control) or exposed to 2 cycles of hyperoxic-hypoxic-hyperoxic treatment (reoxygenation). Viable cells harvested from LTBMC after 2 weeks of culture were counted and presented as mean of 3 independent experiments. *Statistical significance between reoxygenated WT and Fancc^–/– samples at P < .01. (B) Effect of reoxygenation on LTBMC progenitor activity. The colony-forming cell (CFC) activity of WT and Fancc^–/– LTBMC cells was evaluated after hyperoxia (Control) only or 2 cycles of hyperoxic-hypoxic-hyperoxic treatment (Reoxygenation). Data shown represented the total number of colonies (mean ± SD) from 3 independent experiments. *Statistical significance between reoxygenated WT and Fancc^–/– samples at P < .01. (C) Analysis of apoptosis in WT and Fancc^–/– LTBMC cells by quantification of the fraction of cells containing activated caspase 3 (gated in R3). Shown are representative flow cytometric presentations of 3 independent experiments.