MUNC13-4 expression by Western blot analysis in FHL patients. Cell extracts from the T cells of healthy control (C), FHL2, and FHL3 patients (20 μg each) were analyzed by Western blot with anti–MUNC13-4 antibody. The data represent 3 independent experiments. NS indicates nonsense mutation; and MS, missense mutation. The numbers above the lanes correspond to the UPNs of patients listed in Table 2.