IFN-γ production by alloantigen-specific CD8⁺ T-cell lines in response to stimulation with allogeneic B-LCLs. CD8⁺ T-cell lines were generated from the PBMCs of the patients with FHL and healthy controls by stimulation with allogeneic B-LCLs (KIN-LCLs). Responder cells were cocultured with or without KIN-LCLs or TAK-LCLs, which shared no HLA antigens with KIN-LCLs, in the presence or absence of anti–HLA class I monoclonal antibody for 3 days. IFN-γ production was determined by ELISA; the results are mean values (± SD) from triplicate experiments. HLA types of B-LCLs were as follows: KIN-LCL, HLA-A01/30, B13/17, Cw6/-, DRB1^*0701/^*0701; TAK-LCL, HLA-A24/26, B62/-, Cw4/w9, DRB1^*0405/^*0901. NS indicates nonsense mutation; MS, missense mutation; and UPN, unique patient number.