Cross-presentation of HCV-LPs to intrahepatic core-specific CD8+ T cells. Autologous imDCs were loaded with HCV-LPs (50 μg/mL; equivalent to approximately 5 μg HCV-LP core/mL), insect cell control preparation (GUS Ctrl; 50 μg/mL), or core peptide 6 (comprising core aa's 36-53; 10 μg/mL). The pulsed DCs were washed and subsequently cocultured with intrahepatic core-specific CD8+ T cells at a ratio 1:2. After 5 hours of incubation, the cells were stained with antibodies to CD8 and IFN-γ and analyzed by flow cytometry. Percentages of CD8+ T cells that produced IFN-γ in the respective quadrants are indicated on the dot plots. Controls include isotype-control stained CD8+ T cells to determine the quadrant boundaries (not shown).