Figure 2.
Hemin induction of K562 cells. (A) A DNA SATB1 probe ( \(\mathrm{Wt}_{7}^{25}\)
)17 used for EMSA shows a high-molecular-weight band (SATB) from K562 extract. The radiolabeled probe was incubated with no extract (ne, lane 1) or K562 extract before and after hemin induction, indicated in hours (lanes 2-6). Lane 7 contains uninduced K562 cell extract and cold probe as competitor. The large faster-migrating band in lanes 5 to 7 represents nonspecific binding. (B) Western blotting using anti-SATB1 serum and 5 μg protein of K562 cell extract following hemin induction indicated in hours (lanes 2-5) shows the 96-kDa band up to 48 hours after hemin induction. As loading control, β-actin (βac) is also indicated (lanes 6-9). Lane 1 contains molecular weight markers. (C) The γ- and ϵ-globin mRNAs from K562 cells were quantified before and after hemin induction (indicated in hours). Results were normalized to the level of β-actin mRNA. Error bars represent SD from 3 independent measurements.
![Figure 2. Hemin induction of K562 cells. (A) A DNA SATB1 probe (\batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{Wt}_{7}^{25}\) \end{document})17 used for EMSA shows a high-molecular-weight band (SATB) from K562 extract. The radiolabeled probe was incubated with no extract (ne, lane 1) or K562 extract before and after hemin induction, indicated in hours (lanes 2-6). Lane 7 contains uninduced K562 cell extract and cold probe as competitor. The large faster-migrating band in lanes 5 to 7 represents nonspecific binding. (B) Western blotting using anti-SATB1 serum and 5 μg protein of K562 cell extract following hemin induction indicated in hours (lanes 2-5) shows the 96-kDa band up to 48 hours after hemin induction. As loading control, β-actin (βac) is also indicated (lanes 6-9). Lane 1 contains molecular weight markers. (C) The γ- and ϵ-globin mRNAs from K562 cells were quantified before and after hemin induction (indicated in hours). Results were normalized to the level of β-actin mRNA. Error bars represent SD from 3 independent measurements.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/8/10.1182_blood-2004-08-2988/6/zh80080577250002.jpeg?Expires=1724263072&Signature=L6xGqsjdIiQXW1vhM6Z~B7rptKyZd6Vw1M57TdIZG8WBsKIZglrowH728QwavL~wCPm~js8JGqV9VYgaPSfv6ymMcfrcA9NB5qXTPvXyPzjAF31YpbW2fkKVXLgN7B-KbJ2CPag5w2sPh06ZBzsJDbfBhb6dm9YsWOLEhdWeA20OUGUQnC0ViZHQiER7rdBGKNaJ7rMtDBUmxx3kBnjcMQZeSragwGTKPt-iCAsIOXqN0w2T3M-wBGOVepyo6Wsvlr7QylH4-bfiMTHaWEiWDLRde0xPZznR5lnDz0Qbp6PiENNif39zj8tm12aKA-aJ8QHLJ26ZIKQ1sN1Mn~YTfA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
