Figure 4.
Figure 4. CD178- and/or TRAIL-mediated apoptosis of CLL B cells after CD40-ligation. (A) CLL cells from representative patients 1 (▪) or 2 (▨) were cocultured with HeLa-CD154 cells to effect CD40 activation and then tested 24 hours later for their susceptibility to apoptosis induced by 8 hours' coculture with CHO cells (–/–), CHO-CD178 cells (+/–), CHO-TRAIL cells (–/+), or CHO-CD178/TRAIL cells (+/+), as indicated at the bottom of the figure. The bars depict the mean levels of apoptosis observed for 3 independent experiments, as indicated on the left axis. The error bars indicate the standard errors about the mean. For both samples, the mean levels of apoptosis observed upon coculture with CHO-CD178/TRAIL cells were significantly greater than those observed following coculture with CHO, CHO-CD178, or CHO-TRAIL cells (indicated by the bars with the asterisk) (Bonferroni t test ≥ 0.05). (B) Leukemia cells from 9 different patients were cocultured for 8 hours with each of the various CHO effector cells on days 1 (▪), 3 (□), or 5 (▦) after coculture with HeLa-CD154. The bars depict the mean levels of apoptosis that were observed for the 9 different CLL patient samples at the various times when cultured with CHO effector cells as indicated at the bottom of the figure. The error bars depict the standard deviation of the mean. The asterisk highlights the bars representing the mean levels of apoptosis observed in the CLL cell samples when cultured with CHO-CD178/TRAIL cells, each of which were significantly greater than the mean levels of apoptosis observed in the respective CLL cell samples cocultured with CHO cells, CHO-CD178 cells, or CHO-TRAIL cells (P ≥ .05, Bonferroni t test). (C) Unstimulated (▪) or CD40-activated (□) leukemic cells from 9 different patients were cocultured for 8 hours with CHO-CD178/TRAIL effector cells on day 1, day 3, and day 5 after HeLa coculture. The bars depict the mean levels of apoptosis and the error bars the standard deviation of the mean. The asterisks indicate significantly greater apoptosis levels of the CD40 activated versus the unstimulated samples on the respective days (P ≥ .05, Student t test).

CD178- and/or TRAIL-mediated apoptosis of CLL B cells after CD40-ligation. (A) CLL cells from representative patients 1 (▪) or 2 (▨) were cocultured with HeLa-CD154 cells to effect CD40 activation and then tested 24 hours later for their susceptibility to apoptosis induced by 8 hours' coculture with CHO cells (–/–), CHO-CD178 cells (+/–), CHO-TRAIL cells (–/+), or CHO-CD178/TRAIL cells (+/+), as indicated at the bottom of the figure. The bars depict the mean levels of apoptosis observed for 3 independent experiments, as indicated on the left axis. The error bars indicate the standard errors about the mean. For both samples, the mean levels of apoptosis observed upon coculture with CHO-CD178/TRAIL cells were significantly greater than those observed following coculture with CHO, CHO-CD178, or CHO-TRAIL cells (indicated by the bars with the asterisk) (Bonferroni t test ≥ 0.05). (B) Leukemia cells from 9 different patients were cocultured for 8 hours with each of the various CHO effector cells on days 1 (▪), 3 (□), or 5 (▦) after coculture with HeLa-CD154. The bars depict the mean levels of apoptosis that were observed for the 9 different CLL patient samples at the various times when cultured with CHO effector cells as indicated at the bottom of the figure. The error bars depict the standard deviation of the mean. The asterisk highlights the bars representing the mean levels of apoptosis observed in the CLL cell samples when cultured with CHO-CD178/TRAIL cells, each of which were significantly greater than the mean levels of apoptosis observed in the respective CLL cell samples cocultured with CHO cells, CHO-CD178 cells, or CHO-TRAIL cells (P ≥ .05, Bonferroni t test). (C) Unstimulated (▪) or CD40-activated (□) leukemic cells from 9 different patients were cocultured for 8 hours with CHO-CD178/TRAIL effector cells on day 1, day 3, and day 5 after HeLa coculture. The bars depict the mean levels of apoptosis and the error bars the standard deviation of the mean. The asterisks indicate significantly greater apoptosis levels of the CD40 activated versus the unstimulated samples on the respective days (P ≥ .05, Student t test).

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