Binding of purified αVβ3 integrin to immobilized αC monomers and αC(FXIII) or αC(tTG) oligomers. Plastic wells were coated with 20 μg/mL αC monomers (□), αC(FXIII) oligomers (▪), or αC(tTG) oligomers (▦). Binding of purified αVβ3 integrin (20 μg/mL) was measured by ELISA with anti-β3 mAb 25E11 followed by secondary goat anti–mouse IgG coupled with peroxidase. Binding to αC monomers was taken as 100%. Results are the means ± standard deviations of 2 independent experiments performed in triplicates.