Adoptively transferred Id-specific CD8+ T cells cure mice with an established tumor. Mice were vaccinated once with 0.3 × 106 DCs infected with rVV-Id-LAMP1 and rVV-S65L as negative control. Fourteen days after vaccination, mice were killed, and spleens and lymph nodes were harvested, pooled within each group, and depleted of B cells, and CD4 and CD8 cells were positively selected. Mice were injected with 1000 38C13 viable tumor cells subcutaneously (day 0), and 7 days later they received cyclophosphamide as described in “Materials and methods.” Immune T cells were transferred intravenously at day 9 after tumor challenge, and mice were followed for survival and tumor growth. * indicates 38C13; ×, CTX (cyclophosphamides); ▴, rVV-Id-LAMP1 CD4; ⬡, rVV-Id-LAMP CD8; ▪, rVV-Id-LAMP1 CD4 + CD8; ▪, rVV-S65L CD4; ○, rVV-S65L CD8; and ▪, rVV-S65L CD4 + CD8.
