Induction of a Id-specific CD4+ and CD8+ responses by Id-LAMP1 vaccine. Mice were vaccinated once with 0.3 × 106 mature DCs infected with rVV-Id (▴), rVV-Id-LAMP1 (▪), and rVV-S65L (○) as negative control. Fourteen days after vaccination, mice were killed, and splenocytes were stimulated with irradiated 38C13 tumor cells. After 8 days of culture, CD4+ effectors were selected and tested in a 72-hour proliferation assay using as stimulators DCs transfected with mRNAs coding for Id-LAMP1 (A) and S65L (B) or DCs treated (solid line) or untreated (dashed line) with chloroquine and infected with rVV-Id-LAMP1 (C) or rVV-S65L (D). At 5 days of culture, a cytotoxicity assay was performed using as effectors CD8+-selected T cells and as targets the 38C13 (E) or the irrelevant CH27 (F) cell lines.
