Association of WAVE proteins with Triton X-100–insoluble pellets. Platelets were lysed with Triton X-100–EGTA buffer before (R for resting) or after stimulation by TRAP (A for aggregation) or TRAP in the presence of RGDS (N for no aggregation). Lysates were separated by high-speed centrifugation into soluble fractions (soluble) and insoluble (insoluble) pellets. Proteins from each fraction were separated by SDS-PAGE electrophoresis and immunoblotted with anti-WAVE antibodies as indicated.