PML relocalizes into novel microspeckles upon UV radiation. (A) MEFs were UV-irradiated (60 J/m2) and then stained with an antibody against mouse PML. Nuclei were visualized by DAPI (4′,6-diamidino-2-phenylindole). (B) PML is delocalized upon cisplatin treatment. WI-38 cells were treated with cis-pt at 10 μg/mL for 6 hours and subsequently fixed and stained with an antibody against human PML (green). Nuclei were visualized by DAPI. (C-E) Analysis of PML-NB components in UV-irradiated fibroblasts. (C) WI-38 cells grown on coverslip were UV-irradiated (60 J/m2), stained with antibodies anti-PML (green) and anti-SUMO1 (red), and analyzed by confocal fluorescence microscopy. Nuclei were visualized by DAPI (blue). Colocalization of PML and SUMO1 is shown (yellow). (D) WI-38 cells were stained with antibodies against PML (green) and DAXX (red). Colocalization of PML and DAXX is shown (yellow). (E) Cells were stained with antibodies against PML (green) and CBP (red). Colocalization of PML and CBP is shown (yellow).
