G185R mutant does not inhibit neutrophilic differentiation. Growth curve of (A) proliferating and (B) differentiating HL-60 cells transduced with empty MIEG3 vector (red squares and line), WT NE (green triangles and line), or the G185R mutant (blue diamonds and line). (C) Wright-Giemsa stains of WT NE- and G185R-transduced cells show mature neutrophils (arrows) at day 7 of DMSO treatment. (D) Flow cytometric analysis of CD11b expression in transduced cells after culture in DMSO-containing media (1.25% vol/vol) for day 0 (purple shaded curve), day 3 (green curve), and day 7 (pink curve).