Synergistic response of ALL cells to stimulation by CpG ODN and CD40 ligand. Precursor B-ALL cells were incubated for 48 hours either unstimulated or in the presence of CpG 2006, CD40L-expressing fibroblasts, or both 2006 and CD40L. The cell supernatants were then analyzed for the presence of IL-6, IL-8, and IL-10. IL-12p70 was not detectable under any of the assay conditions. The results are shown as the mean ± standard deviation for 2 independent experiments.