Responses of precursor B-lineage ALL cell lines to CpG stimulation. (A) The cell lines were incubated for 48 hours in the absence (untreated) or presence of 6 μg/mL CpG ODN 2006 or control ODN 2137, then analyzed for costimulatory molecule expression. Quadrants were drawn using isotype control antibody–stained cells. The percentage of cells in each quadrant is shown. A representative of 3 independent experiments is shown. (B) Cell lines were cultured for 24 hours in absence (▦) or presence (▪) of CpG 2006 and proliferation measured by 3H-thymidine incorporation. The results are expressed as the average ± SE of 2 independent experiments. (C) RS4;11 (▦) or 697 (▪) cells were incubated for 48 hours in the presence or absence of the indicated CpG ODN, and analyzed for CD40 expression. Results are expressed as the change in the percentage of CD40-expressing cells following CpG treatment. A representative of 2 independent experiments is shown.
