Rescue from apoptosis in unprimed naïve Tg8.4 and Tg8.7 T cells. (A) Cell cycle analysis of purified T cells following 3 days culture in the presence of immobilized carrier protein (FgG), antigen (FgG-TNP), or anti-CD3 antibodies. Cells were fixed and stained with propidium iodide to analyze their DNA content. Cell cycle histograms fitted to the different regions were used to determine the proportion of cells in the various cell cycle phases. Region M2 represents cells in G1, whereas regions M3 and M4 show cells in S and G2/M phases, respectively. Hypodiploid (apoptotic) cells are indicated in region M1. Results are representative of 3 different experiments with 3 mice/group. (B) Kinetics of bcl-XL protein induction following stimulation with FγG (I), FγG-TNP (II), or anti-CD3 (III) as determined by immunoblotting.
