CXCL10 plus CCL8 reverts the antiadhesive activity also of VEGF and bFGF. HUVECs were either left untreated or pretreated for 3 days with VEGF or bFGF before addition of TNF-α and adhesion assay. At the time of the HL-60 cell adhesion assay, the indicated recombinant chemokines (10 ng/mL each) were added to the cultures. TNF-α-induced HL-60 cell adhesion to HUVECs was set as 100%. Results are expressed as means ± SD of 4 independent experiments, each performed in triplicate.