Figure 6.
![Figure 6. Specificity of the NF-κB–binding site. EMSAs were performed using nuclear protein from HUVECs and biotin-labeled probes (WT [wild-type] oligo, biotin or Mut oligo, biotin) as described in Figure 5. Symbols above each lane (+/-) denote the presence or absence of various reagents. For competition experiments (lanes 4 and 6), a 50-fold excess of unlabeled oligo (WT oligo or Mut oligo) was used. Data are representative of at least 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/9/10.1182_blood-2004-07-2617/6/zh80090577870006.jpeg?Expires=1730492703&Signature=Wr5rJMkrIjBkBGl3BLg18t8BByhZZzIloc~sm8bkHqK8qLI6aixTrV7svKa8TqcY8JoLg~sUg1-z3JCzXQoGM3HtCNzXaW4mnL9Z7uNRXJffXXUeM4KiZmFVFzbrqBp51vi0Q93nJmfPXMMEVSgGwxQ8ZhuBIkwQ5FwuQ88GAwT7TjPmUnBSCc~yEC7HoI73uVC9lXyv6dtp1lpD1554s2kMI3Es8RDsWrXAh2~lU6NYRUZrs~1~qyjIFIGoJZEex668WXbdMZZvt1Qc7mYeAgGu9lYeneYbLLCrDkQACLzuVmX07PHK-vFTWgpXCp3TxkAmbllJ4L3cyCZD1kA-sQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Specificity of the NF-κB–binding site. EMSAs were performed using nuclear protein from HUVECs and biotin-labeled probes (WT [wild-type] oligo, biotin or Mut oligo, biotin) as described in Figure 5. Symbols above each lane (+/-) denote the presence or absence of various reagents. For competition experiments (lanes 4 and 6), a 50-fold excess of unlabeled oligo (WT oligo or Mut oligo) was used. Data are representative of at least 3 independent experiments.
