Imatinib does not affect c-fms expression by an M-CSF–dependent cell line. (A,B) FDC-cfms cells were starved for 1 hour in the presence of imatinib, and examined for c-fms expression by Western blotting (IC indicates isotype control) or (C) flow cytometry. Immunoprecipitates were examined for c-fms protein expression, with the 170-kD band (□) representing the mature c-fms protein, and the 130-kD band representing the immature, nonglycosylated form (▪) (A,B). Results are representative of 3 individual experiments. MFI indicates mean fluorescence intensity, and isotype matched controls are represented by unfilled histograms (C).