sPLA2 treatment of DCs enhances their stimulatory capacity in the allogeneic MLR: proliferative responses. Day-5 DCs were treated in the presence of FCS or LPD-FCS for 48 hours with sPLA2 alone (10 μg/mL) or in combination with TNF-α (100 U/mL) plus IL-1β (1 ng/mL). Cells were washed and used as stimulators of 3 different preparations of allogeneic PBMCs depleted of CD14⁺ cells. Proliferation was determined as [³H] thymidine incorporation. Results from 1 of 2 almost identical experiments are shown. Mean values of triplicate measurements ± SD are shown. LPD indicates lipoprotein-deficient.