DC-culture supernatants lack sPLA2 activity. Day-5 DCs were treated as indicated with TNF-α (1000 U/mL), IL-1β (5 ng/mL), and PGE2 (1 μM); sPLA2 was added at increasing concentrations (0.1 μg/mL-10.0 μg/mL). After a 48-hour culture supernatants were harvested and assessed for sPLA2 activity using a commercial sPLA2 assay kit. Results from 1 of 3 similar experiments are shown. mOD₄₁₄ = (optical density at 414 nm per minute) × 10^-3. Mean values of triplicate measurements ± SD are shown.