Inhibition of the PI-3K/Akt pathway blocks SK-induced cell survival. ECs^SK (▪) and ECs^EV (□) were treated with 10 mM LY294002 (LY) to block the PI-3K/Akt pathway and 20 mM UO126 (UO) or 20 mM PD98059 (PD) to block the MAPK pathway. A vehicle control of equivalent concentration of dimethyl sulfoxide (DMSO) is indicated. The figure shows cell survival by the pooled data of 8 observations from 2 separate experiments, adjusted to day 0 = 1. Bars represent 95% confidence intervals. *P less than .001 compared with corresponding untreated cells overexpressing SK or EV at day 2. (B) Western blot measuring phosphorylated Akt (p-Akt) and total Akt in basal conditions (HM) and after 6 hours of serum deprivation (SF) in ECs^SK and ECs^EV. The effect of 20 μg/mL of rabbit polyclonal anti–PECAM-1 antibody (RP) added at plating under SF conditions is shown. (C) The pooled data of the quantification of phosphorylated Akt compared with total Akt from 4 separate experiments performed as in panel B for ECs^SK (▪) and ECs^EV (□). Bars represent SEM. *P less than .05 of untreated ECs^SK versus ECs^SK treated with RP.