Figure 5.
Figure 5. Immunofluorescence staining of muscle sections of the tibialis anterior of hemophilia B dogs injected 2 (dog F57) or 3 (dog D99) years earlier with AAV-CMV-canine F.IX by isolated limb perfusion. (A, D). Fluorescence of rhodamine (red) showing presence of canine F.IX in muscle fibers of ILP injected dogs F57 (A) or D99 (D). (B, E) Fluorescence of fluorescein isothiocyanate (FITC; green) showing presence of heparan sulfate proteoglycan (HSPG) in fibers of tibialis anterior of dog F57 (B) or D99 (E). (C, F) Simultaneous excitation of both fluorescence tags. (G) Fluorescence of rhodamine showing absence of canine F.IX expression in muscle biopsy from dog E59 (infusion of vector by peripheral vein). (H) Hematoxylin and eosin staining of same samples of dog (F57). For all panels, original magnification ×100.

Immunofluorescence staining of muscle sections of the tibialis anterior of hemophilia B dogs injected 2 (dog F57) or 3 (dog D99) years earlier with AAV-CMV-canine F.IX by isolated limb perfusion. (A, D). Fluorescence of rhodamine (red) showing presence of canine F.IX in muscle fibers of ILP injected dogs F57 (A) or D99 (D). (B, E) Fluorescence of fluorescein isothiocyanate (FITC; green) showing presence of heparan sulfate proteoglycan (HSPG) in fibers of tibialis anterior of dog F57 (B) or D99 (E). (C, F) Simultaneous excitation of both fluorescence tags. (G) Fluorescence of rhodamine showing absence of canine F.IX expression in muscle biopsy from dog E59 (infusion of vector by peripheral vein). (H) Hematoxylin and eosin staining of same samples of dog (F57). For all panels, original magnification ×100.

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