Figure 4.
Effect of iron on IFN-γ–induced activation of STAT1 in ST4 T cells. ST4 cells were cultured in complete medium, with or without 10 μM FeSO4 or DFO, and in serum-free medium with or without FeSO4. After 24 hours cells were recovered and treated with 1000 U/mL IFN-γ for 15 minutes. STAT1 activation was evaluated by Western blot analysis of nuclear cell extracts with anti–phospho-Tyr701-STAT1 polyclonal Ab. Membranes were subsequently probed with an anti-STAT1 antibody to confirm equal protein loading in each lane of the gel. The experiments were performed independently at least 3 times.
