Effect of iron on expression of the IFN-γR chains in human malignant T cells. Flow cytometric analysis of IFN-γR1 and IFN-γR2 surface expression in ST4 malignant T cells cultured in complete medium (A-B), in complete medium with 10 μM FeSO4 (C-D), in complete medium with DFO (E-F), in serum-free medium (G-H), and in serum-free medium with FeSO4 (I-J). The histogram represents the expression of IFN-γR1 (left panels, gray histograms), IFN-γR2 (right panels, gray histograms), or background of mouse IgG1 negative control (open histograms). Shown are the results of 1 representative experiment of 3 independently performed. Boxed results show percentage of positive cells calculated by subtracting the positivity of nonspecific fluorescence detected with isotype-matched control Ig from that obtained with specific fluorescence.
