Cytokine and chemokine secretion by mono-DCs upon stimulation through hOSCAR. (A) Immature mono-DCs were stimulated with plastic-coated isotype controls (MOPC21, anti-CD13), anti-hOSCAR F(ab′)2 and whole mAb, soluble anti-hOSCAR, and LPS. As stated, polymixin B was added to the culture to block LPS activity. After 24 hours, supernatants were tested by ELISA for IL-8/CXCL8 secretion. Data are mean ± SD of triplicate samples from 1 representative experiment out of 3 performed with similar results. Statistical significances of *P < .001 are given by comparison to values obtained with the negative control anti-CD13. (B) Mono-DCs were stimulated with plastic-coated isotype control MOPC21, anti-hOSCAR, and LPS. After 2 hours of stimulation, cells were harvested and lysed. The transcripts coding for chemokines were analyzed by real-time PCR using SYBR-Green. Data are given after normalization of Ct (cycle threshold) value of 1 gene with the expression of GAPDH transcripts. Data are mean ± SD of triplicate samples from 1 representative experiment out of 3 performed with similar results. *P < .01 are given by comparison to values obtained with the negative control MOPC21.
