Stimulation through hOSCAR decreases the macropinocytic/endocytic activity of mono-DCs. After 48 hours of stimulation with coated antibodies (isotype controls, MOPC21 and anti-CD13 or anti-hOSCAR) or with 10 ng/mL LPS, and 20 ng/mL TNF, mono-DCs were incubated at 37°C for 30 minutes in the presence of FITC-dextran. The cells were then harvested, stained with anti-CD86–PE, and immediately analyzed by flow cytometry. Numbers in the corners correspond to the percentage of cells in each panel. Data shown are representative of 4 independent experiments.