Stimulation through hOSCAR synergizes with TLR ligands to increase the expression of maturation markers expressed by mono-DCs. Mono-DCs were stimulated with coated MOPC21 (□), anti-hOSCAR (▪), and/or LPS, R-848, and poly(I:C) at the indicated dose levels. After 24 hours of culture, cells were analyzed by flow cytometry for the indicated markers. The data are expressed as ΔMFI, the mean fluorescence intensity minus the fluorescence detected with isotype control. The results presented are representative of 3 independent experiments.