American Society of Hematology

Figure 3.

$Figure 3. Role of CD47 for platelet half-life in circulation. Platelets were isolated from CD47+/+ (▪), CD47+/- (□), or CD47-/- (⬡) C57BL /6 mice, labeled with the cell tracker CMFDA, and injected into (A) recipient mice of the same genotype or (B) CD47+/+ mice. At the times indicated, 5 μL blood was sampled from a tail vein of the recipients and analyzed by flow cytometry for the fraction of fluorescent platelets of the total recipient platelets. Data were normalized to the level at 10 minutes (CD47-/- to CD47+/+) or 2 hours after injection. Data are means ± SEMs for 4 to 5 mice in each group.$

Role of CD47 for platelet half-life in circulation. Platelets were isolated from CD47^+/+ (▪), CD47^+/- (□), or CD47^-/- (⬡) C57BL /6 mice, labeled with the cell tracker CMFDA, and injected into (A) recipient mice of the same genotype or (B) CD47^+/+ mice. At the times indicated, 5 μL blood was sampled from a tail vein of the recipients and analyzed by flow cytometry for the fraction of fluorescent platelets of the total recipient platelets. Data were normalized to the level at 10 minutes (CD47^-/- to CD47^+/+) or 2 hours after injection. Data are means ± SEMs for 4 to 5 mice in each group.

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