Figure 6.
![Figure 6. The M1.2 peptide deduced from the signal sequence of the MUC1 protein is presented TAP independently. To analyze if a functional TAP is required for the presentation of HLA class I epitopes, autologous DCs were cotransfected with MUC1 and ICP47 RNA (MUC1 alone, ×; ICP47 alone, ♦; both, ▴) and used as targets for the recognition by MUC1-specific CTLs (M1.1 [A] and M1.2 [B]). DCs pulsed with the synthetic cognate MUC1 peptide (▪) or electroporated with irrelevant EGFP RNA (×) were included as controls.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/8/10.1182_blood-2004-09-3556/6/zh80080576850006.jpeg?Expires=1724247247&Signature=lN~ZkDtjRRLW9tb~zyRhbJOunZjxRKvMCSZzArNEUZMjLld7CKI12yt3PKig1l9J5gilG~19yQ~UGPOhp5B~Vnpx999ltpYT1KI-0JFb5hUhLWHaLOAsAgvoBDVPihDF8h4RGaSADiAVdxmUNxO8RhcJ-QHq5~11y4w9X1P6hLiB9vs~LancSLnTNCMa08RmrPDIcnxZrUJHAKNlUUtmxBiHoNcWsmwCDDKrKjDroGP~KDipwsdEncagS9cqvcXHwhKL19KCfkwSRsmkKh-dNqUQIhlH-b7RjZi05ihuHQEdQIs4iPyfSwfAdlMVKxYId-DQ~O4agfd8snEpbvgXQA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The M1.2 peptide deduced from the signal sequence of the MUC1 protein is presented TAP independently. To analyze if a functional TAP is required for the presentation of HLA class I epitopes, autologous DCs were cotransfected with MUC1 and ICP47 RNA (MUC1 alone, ×; ICP47 alone, ♦; both, ▴) and used as targets for the recognition by MUC1-specific CTLs (M1.1 [A] and M1.2 [B]). DCs pulsed with the synthetic cognate MUC1 peptide (▪) or electroporated with irrelevant EGFP RNA (×) were included as controls.
