Figure 1.
![Figure 1. Induction of MUC1-specific CTL responses in vitro. DCs generated from adherent PBMNCs of HLA-A*02–positive donors in the presence of GM-CSF, IL-4, and TNF-α were pulsed with synthetic peptides and used to generate a CTL response in vitro. Cytolytic activity of induced M1.1- (A) and M1.2- (B) specific CTLs was analyzed in a standard 51Cr-release assay using autologous DCs pulsed with the cognate peptide (M1.1 in A [♦]; M1.2 in B [▪]) or an irrelevant peptide (M1.2 in A [□]; M1.1 in B [⋄]; HIV peptide [▵] in both panels) as targets. E/T ratio, effector-target ratio.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/8/10.1182_blood-2004-09-3556/6/zh80080576850001.jpeg?Expires=1724247534&Signature=tQDvjRfBKxyvlIbkMdvuIhPS7C2X9e1WrD4zIA4bfW9m4AYTliZqbUsSbufWGde0hxUHiBy-Rsomd56B4di1CzilHBUalXZNiOegBQZHOnLL13E5pUhEjXS1IOs1cW5FKBu0QeO2IaoIBE~i4UNJ24oWkXYPxSSCsNoV-4e~D1rb-UCM9Ja~yBt40xynptMxpAut7DSbwnpirvZHi6KVXTFcUiy2GbLAPRYEMMB4XE-QGODsKp1Tfzqtq~~SAIAVX7ZjOz24RpRX4Ll2-4AzPcfw0RSw2Gfa7X~mCZB4GwUuVMJdep9qIoTOyHxkeqwk~LS7~WYR2EOeGoqqofiDug__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Induction of MUC1-specific CTL responses in vitro. DCs generated from adherent PBMNCs of HLA-A*02–positive donors in the presence of GM-CSF, IL-4, and TNF-α were pulsed with synthetic peptides and used to generate a CTL response in vitro. Cytolytic activity of induced M1.1- (A) and M1.2- (B) specific CTLs was analyzed in a standard 51Cr-release assay using autologous DCs pulsed with the cognate peptide (M1.1 in A [♦]; M1.2 in B [▪]) or an irrelevant peptide (M1.2 in A [□]; M1.1 in B [⋄]; HIV peptide [▵] in both panels) as targets. E/T ratio, effector-target ratio.
