Trafficking of the K119TmATS-GFP protein is brefeldin A (BFA) sensitive. (Ai-ix) Live cells. GFP fluorescence (i,iv,vii); bright-field image (ii,v,viii); overlay (iii,vi,ix). Normal trafficking of the K119TmATS-GFP chimera was observed in the absence of BFA (-) or after washing, whereas BFA (+) treatment inhibited trafficking. (Ax-xiii) Immunofluorescence microscopy of fixed BFA-treated cells. (x) αGFP; (xi) αBiP; (xii) DAPI; (xiii) merge of subpanels x-xii. The chimera colocalizes with the ER marker BiP after incubation with BFA. (B) Brefeldin A treatment of 3D7-TmATS transfectants. (Bi-vi) Live cells in the presence (+) and absence (-) of BFA. GFP fluorescence (i,iv); phase-contrast images (ii,v); overlays (iii,vi). (Bvii-x) Immunofluorescence microscopy of fixed BFA-treated cells. (vii) αGFP antibody; (viii) αBiP antibody; (ix) DAPI; (x) merge of subpanels vii-ix.