Responsiveness of PELs dually infected with HHV-8 and EBV to valproate and antivirals. JSC-1 cells were incubated in the absence or presence of valproate. Where indicated, ganciclovir or PFA was present through the incubation at the indicated concentration (μM). (A) The total number of cells at each time point in the absence (♦) and presence (▪, 0.6 mM; ▵, 1.2 mM) of valproate. (B) The percentage of cells that were apoptotic at each time point as assessed by flow cytometric analysis of TUNEL-positive cells. □ indicates absence of valproate; ▪, 0.6 mM valproate; ▨, 1.2 mM valproate. (C) The relative amount of infectious virus in conditioned medium at each time point based on β-galactosidase produced by the T1H6 reporter cell line. □ indicates absence of valproate; ▪, 0.6 mM valproate. Values shown are the mean ± SD from 3 replicate determinations.