Localization of HGAL protein and specificity of anti-HGAL monoclonal antibody. (A) Confocal immunofluorescence microscopy using a FITC-conjugated anti-V5 antibody in transfected HeLa cells shows that the HGAL protein is localized to the cytoplasm (green fluorescence). The nuclei are counterstained with an orange Hoechst dye (original magnification, ×1000). (B) Western blot analysis using the anti-HGAL monoclonal antibody shows a specific band corresponding to HGAL protein expression in Raji cells (positive control) and in HeLa cells stably transfected with pcDNA3.1 HGAL-V5 but not in native or mock-transfected HeLa cells.