Combination effect of OSU-03012 and imatinib mesylate at different concentrations. Combination effect of OSU-03012 and imatinib mesylate at different concentrations on the state of Bcr-Abl expression (A) and Akt phosphorylation (B) in Ba/F3p210^E255K and Ba/F3p210^T315I cells. The immunoblots are representative of 3 independent experiments. Bars represent means ± SD (n = 3). No appreciable changes in Bcr-Abl expression levels were noted. However, a decreasing trend of p-Akt with increasing doses of imatinib mesylate and OSU-03012 in combination with 5 μM OSU-03012 and 5 μM imatinib mesylate, respectively.