Genomic changes and survival in MCL. (A) FISH analysis using CCND1 flanking probes in patient MCL047 showing 11q13 amplification. (B) FISH analysis of patient MCL114 (male) showing homozygous deletion of the Kallmann syndrome locus. Green indicates centromeric probe for chromosome X; red, probe for KAL1 gene locus at Xp22.31, which is not detected in all but one of the interphase nuclei. A Zeiss Axioplan fluorescence microscope with Vysis imaging software was used. (C) Array CGH and survival in MCL. OS of patients with MCL according to the deletions of chromosomes p21, 9q21-q22, and 17p13.1 at TP53 and blastoid versus classical cytology. (D) Examples of array CGH profiles of 2 patient samples with MCL. (Top) Case no. MCL009 shows few genomic alterations, including deletions of 1p21, 6q22-q27, and 8p12-p23.3 and gains of 7p11-p22 and 8q12-q24. Survival time for this patient was 115 months since diagnosis. (Bottom) The array CGH profile of case no. MCL047 showing a large number of genomic changes, including deletions of 1p21 and 9p21.3 at P16/INK4a gene locus and 9q21-q22 and 17p13.1 at TP53 gene locus, along with high-level amplification within BCL1 gene locus. Survival time for this patient was 1 month since diagnosis.