Etoposide triggers mitochondrial perturbations in the Raji BL cell line. Dissipation of mitochondrial membrane potential (A) and release of cytochrome c (B) in etoposide-treated Raji BL cells was evaluated by flow cytometry and immunoblotting of cytosolic extracts versus mitochondrial pellets (30 μg per lane), respectively. CCCP was used as a positive control for membrane depolarization. Jurkat cells were included for comparison in panels A and B. Results are representative of at least 3 independent experiments.