Findings for peripheral blood of knock-in mice. (A) Peripheral blood cell counts of the mutant mice are indicated by the height of the columns. Lines above the columns indicate standard deviations. WBC indicates white blood cell count; RBC, red blood cell count; and PLT, platelet count. (B) Bar graphs showing differences of peripheral leukocytes from knock-in and control mice. Twenty mice were examined for each mutation, and means of the results are shown. Lymphoid population in peripheral blood of AML1-3 mice is reduced. Dark blue indicates lymphocytes; green, monocytes; pink, eosinophils; light blue, seg (segmented neutrophils); and white, stab (stab neutrophils). (C) Flow cytometric analysis of peripheral T- and B-lymphoid populations, showing no marked differences in the composition of T- and B-cell populations for the mutant and control animals. Six mice were analyzed for each of the genotypes. Numerals below the charts show means and standard deviations for single-positive (SP) populations. (D) Microscopic appearance of representative blood cells from mutant and control animals, showing no marked abnormalities in granulocytes (top row), lymphocytes (middle row), or monocytes (bottom row). Smear samples were stained with May-Grünwald-Giemsa, and the photographs were taken by using a microscope (BH-2, Olympus) equipped with a digital camera (C-5050 Zoom, Olympus). Original magnification, × 300.