Figure 6.
Northern blot analysis and flow cytometric analysis of FL5.12 cells. (A) Northern blot analysis for the IL-3 receptor. Total RNA was extracted from FL5.12 cells that had been transfected with the empty vector (lanes 1-4) or pMT-TEF (lanes 5-8) and cultured in the presence of IL-3 with Zn for the indicated periods of time. The blot was hybridized with mouse cDNA probes specific for IL-3 receptor β chains and α chain. The 28S rRNA visualized with ethidium bromide staining is shown in the bottom panel. (B) Flow cytometric analysis for surface expression of the IL-3 receptor. FL5.12 cells transfected with the empty vector, pMT–E2A-HLF, pMT-TEF, or pMT-TEF/BX were cultured in IL-3–containing medium in the presence or absence of Zn for 24 hours. Cells were analyzed with the specific antibodies for mouse βIL3, βC, or α chains. Dotted or solid lines indicate the histograms of control staining, and filled curves indicate those of specific antibodies.

Northern blot analysis and flow cytometric analysis of FL5.12 cells. (A) Northern blot analysis for the IL-3 receptor. Total RNA was extracted from FL5.12 cells that had been transfected with the empty vector (lanes 1-4) or pMT-TEF (lanes 5-8) and cultured in the presence of IL-3 with Zn for the indicated periods of time. The blot was hybridized with mouse cDNA probes specific for IL-3 receptor β chains and α chain. The 28S rRNA visualized with ethidium bromide staining is shown in the bottom panel. (B) Flow cytometric analysis for surface expression of the IL-3 receptor. FL5.12 cells transfected with the empty vector, pMT–E2A-HLF, pMT-TEF, or pMT-TEF/BX were cultured in IL-3–containing medium in the presence or absence of Zn for 24 hours. Cells were analyzed with the specific antibodies for mouse βIL3, βC, or α chains. Dotted or solid lines indicate the histograms of control staining, and filled curves indicate those of specific antibodies.

Close Modal

or Create an Account

Close Modal
Close Modal