Effect of CYC202 dose on apoptosis and survival of B-CLL cells in vitro. Dose response of B-CLL tumor cells to CYC202 shown by annexin V assay. Percentage of (A) apoptotic and (B) viable cells following 0, 24, 48, and 72 hours of treatment. ♦ and bold solid line indicate no drug (n = 26); ▪ and thin solid line, 1 μg/mL (n = 24); ▴ and dashed line, 2.5 μg/mL (n = 19); and • and dotted line, 5 μg/mL (n = 26) CYC202. Graphs represent mean proportion of apoptotic cells at each time point, and error bars represent 95% confidence intervals.