Compound effects of germline deletion of Pim-1, Pim-2, and Akt-1. (A, left) Cell size measurements are depicted from IL-3–dependent bone marrow cultures from wild-type (WT), Pim-1–null/Pim-2–heterozygous/Akt-1–heterozygous (Pim-1^KO/Pim-2^Het/Akt-1^Het), Pim-1–null/Pim-2–heterozygous/Akt-1–null (Pim-1^KO/Pim-2^Het/Akt-1^KO), Pim-1–heterozygous/Pim-2–null/Akt-1–null (Pim-1^Het/Pim-2^KO/Akt-1^KO), Pim-1–null/Pim-2–null/Akt-1–heterozygous (Pim-1^KO/Pim-2^KO/Akt-1^Het), and Pim-1–null/Pim-2–null/Akt-1–null (Pim-1^KO/Pim-2^KO/Akt-1^KO) animals. Data represent mean ± SD of 3 independent samples of live G1 cells. (Right) Lysates generated from these cells were probed for the expression of Pim-1, Pim-2, Akt-1, and Actin. (B) Cell accumulation after 48 hours of culture is shown for the cells described in panel A. Live cells were seeded in fresh IL-3 containing media at 10⁵ cells/mL, as indicated by the dashed line, after synchronization by 12 hours of IL-3 withdrawal. Data represent mean ± SD of triplicate samples. (C) Cell cycle profiles from the cells described in panel B 24 hours after plating. The percentage of cells with greater than G1 DNA content is indicated.