Pim-2 deficiency sensitizes cells to rapamycin treatment. (A) Primary IL-3–dependent bone marrow cultures were established from Pim-1/Pim-2–deficient (1^KO/2^KO), Pim-2–deficient (1^WT/2^KO), Pim-1–deficient (1^KO/2^WT), and wild-type littermate (1^WT/2^WT) mice. Lysates were probed for the expression of Pim-1, Pim-2, and Actin. Cells were deprived of IL-3 for 12 hours and then stimulated for the number of hours indicated before lysis. (B) Cell number versus days in culture is shown for IL-3–dependent primary bone marrow cultures from the mice described in panel A, grown in the absence (-Rap) or presence (+Rap) of 20 nM rapamycin. Cells were seeded at a concentration of 50 000/mL at the beginning of the experiment. Data indicate the mean ± SD of triplicate samples.