SDF-1 promotes the growth and survival of CFU-F. The total number of CFU-F colonies derived from MACS/FACS-solated STRO-1^bright BMMNCs plated in serum-free media in the presence of different cytokine combinations was enumerated. Recombinant human PDGF-BB, SDF-1α, and α-interferon 2a were used at the optimal concentrations 5 ng/mL, 30 000 IU/mL, and 30 ng/mL, respectively. The data represent the mean values ± standard errors of triplicate wells. Similar results were obtained by using 3 different bone marrow aspirates. Statistical significance (P < .01) was determined by using one-way ANOVA for all treatments. The Fisher test was then used to determine the differences between all groups. Significant differences (P < .05) were found between all treatments compared with PDGF-BB alone (^*), and PDGF + IFN (interferon) verses PDGF + SDF-1 + IFN (^**) at a significance of P < .05.