PHZ-induced anemia reactivates podocalyxin expression in adult erythroid cells. (A) Representative FACS histograms showing PCLP1⁺ cells in BM of adult mice 3 days after injection with either PBS (left) or PHZ (60 mg/kg; right). Cells were stained with PCLP1 (black line) or control mAb (gray line) and counterstained with Alexa-fluor 488–conjugated antirat IgG. (B-C) Coexpression of podocalyxin with hematopoietic progenitor and erythroid markers on BM cells from PBS- and PHZ-injected mice 2 days after the first injection. BM (B) and SPL (C) cells were stained with anti-PCLP1 as in panel A and counterstained with biotinylated Ter119 plus streptavidin-PE or c-kit–PE. Percentages are determined according to quadrant markers.