Figure 6.
![Figure 6. Azaspirane inhibits MM cell growth, as well as IL-6 and VEGF secretion, triggered by tumor cell binding to BMSCs. MM.1S cells, BMSCs, or both MM.1S cells and BMSCs were cultured for 48 hours in the presence of DMSO control (□) or with 0.6 μM(▦), 1.25 μM(▨), and 2.5 μM(▪) azaspirane (A). DNA synthesis was assessed by [3H]-thymidine uptake, and data represent mean (± SD) of quadruplicate cultures. IL-6 (B) and VEGF (C) levels in culture supernatants were measured by ELISA. *P < .01.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/11/10.1182_blood-2004-09-3794/6/zh80110578950006.jpeg?Expires=1724962144&Signature=jpFe7zWXUlt66N7DpxQXlG51ZupcaRxd6WfiOYYsJLlpZLm95xTlCV53x883lvzpJka3HIDdI4~FW0TWPx3OFbl6mY6MebxaM56WOqS-IMJnXMYZoAzUdC~kuykM~s3x8vjygoXAkHAfHWtVH39r5PE34PMIcboKIns99Bi-s-uf3lxnx5JSL8zT8q7oCMT4V24e1dhLp7ZG3Lcd3ctY7fLpu43~URAJvGpWmX~PQFohkqQhUr5l~O9mvWxrmJd2HaYZ2Zim63BTylslCWBPsOstjEH--mY02~Z59-qlRS7wyo5dtFt1xV3Iklk947q6t27-I6~5W-QBCGOujccp4A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Azaspirane inhibits MM cell growth, as well as IL-6 and VEGF secretion, triggered by tumor cell binding to BMSCs. MM.1S cells, BMSCs, or both MM.1S cells and BMSCs were cultured for 48 hours in the presence of DMSO control (□) or with 0.6 μM(▦), 1.25 μM(▨), and 2.5 μM(▪) azaspirane (A). DNA synthesis was assessed by [3H]-thymidine uptake, and data represent mean (± SD) of quadruplicate cultures. IL-6 (B) and VEGF (C) levels in culture supernatants were measured by ELISA. *P < .01.
