Azaspirane inhibits phosphorylation of JAK2/STAT3 and Akt but not ERK1/2. MM.1S cells were pretreated with azaspirane (5 μM) for 2 hours and 4 hours. Cells were then stimulated with IL-6 (20 ng/mL) or TNF-α (10 ng/mL) for 10 minutes. Whole-cell lysates were subjected to Western blotting using anti–phospho-ERK/ERK, phospho-Akt/Akt, phospho-STAT3/STAT3, phospho-JAK2, phospho-IκBα/IκBα, phospho-NFκB p65/NFκB p65, and α-tubulin Abs (A); MM.1S cells were treated with azaspirane (5 μM) for 4 hours and 8 hours. Whole-cell lysates were subjected to Western blotting, using anti–Mcl-1 and α-tubulin Abs (B).